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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: SORBS2 as a molecular target for atherosclerosis in patients with familial hypercholesterolemia

Fig. 5

SORBS2 silencing inhibits activation of NF-κB. A–C Si-SORBS2 treatment inhibited NF-κB signaling in THP1-derived macrophages. Western blotting was performed to evaluate p-IκB-α, IκB-α, p-p65, p65, and GAPDH protein expression levels in macrophages treated with or without Si-SORBS2. D p65 subunit protein levels in the cytoplasm and nucleus were also detected by confocal microscopy. p65 nuclear localization was visualized with an anti-p65 (green) antibody in THP-1 derived macrophages pretreated with Si-SORBS2 followed by OxLDL for 24 h. DAPI (blue) was used as a nuclear marker. The histogram reports mean ± SEM of densitometric scans of protein bands from three experiments (normalized by comparison with GAPDH). * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with the control group (no Ox-LDL treatment); #P < 0.05, ##P < 0.01, ###P < 0.001 compared with the corresponding controls (OxLDL at 50 µg/mL). Scale bar, 25 μm (400 ×)

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