Fig. 4
From: SORBS2 as a molecular target for atherosclerosis in patients with familial hypercholesterolemia
NLRP3 expression and ASC speck formation in THP-1 derived macrophages. A NLRP3 expression was visualized by immunofluorescence analysis with an anti-NLRP3 (green) antibody in THP-1 macrophages pretreated with or without Si-SORBS2 followed by Ox-LDL (50 µg/mL) for 24 h. DAPI (blue) was used as a nuclear marker. Scale bar, 25 µm. B ASC speck formation was visualized by immunofluorescence analysis with an anti-ASC (green) antibody in THP-1 macrophages pretreated with or without Si-SORBS2 followed by OxLDL (50 µg/mL) for 24 h. DAPI (blue) was used as a nuclear marker. Scale bar, 25 μm (400 ×)