Fig. 4

Overexpression of JAK1 promotes the expression of miR-155 in AD. A The expression level of JAK1 in HaCaT cell line following oe-JAK1 treatment was detected by RT-qPCR (* p < 0.05 vs. vector). B JAK1 protein level in HaCaT cell line following oe-JAK1 treatment was detected by Western blot analysis. C The expression levels of miR-155, Drosha, DGCR8 and Dicer1 in HaCaT cell line following oe-JAK1 treatment were detected by RT-qPCR (* p < 0.05 vs. vector). D The protein levels of Drosha, DGCR8 and Dicer1 in HaCaT cell line following oe-JAK1 treatment were detected by Western blot analysis. E The expression of JAK1 in HaCaT cell line following sh-JAK1 treatment was detected by RT-qPCR (* p < 0.05 vs. sh-NC). F JAK1 protein level in HaCaT cell line following sh-JAK1 treatment was detected by Western blot analysis. G The expression level of miR-155, Drosha, DGCR8 and Dicer1 in HaCaT cell line following sh-JAK1 treatment was detected by RT-qPCR (* p < 0.05 vs. sh-NC). H The protein expression levels of Drosha, DGCR8 and Dicer1 in HaCaT cell line following sh-JAK1 treatment were detected by Western blot analysis. I The expression levels of miR-155, Drosha, DGCR8 and Dicer1 in skin tissues of mice with AD induced by PA or MC903 were detected by RT-qPCR (n = 10, * p < 0.05 vs. control). J Western blot analysis was used to detect the protein levels of Drosha, DGCR8 and Dicer1 in skin tissues of mice with AD induced by PA or MC903. Cell experiment was repeated for three times