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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: TPI1 activates the PI3K/AKT/mTOR signaling pathway to induce breast cancer progression by stabilizing CDCA5

Fig. 4

TPI1 interacts with CDCA5 and stabilizes CDCA5 protein. A Clustering analysis of 61 genes co-expressed with TPI1 by STRING tools. Relatively important module was highlighted with Cytoscape tool. B Hub genes were identified using cyto-Hubba tool kits in Cytoscape. C Kaplan–Meier plotter described relationship of differentially expressed TPI1/CDCA5 with OS (based on log-rank test). High levels of TPI1 and CDCA5 were associated with shortened OS in TCGA. D Western blot and qRT-PCR used to quantify expression of CDCA5 in TPI1 knockdown vs. overexpression cells. CDCA5 protein level, but not mRNA level was changed (NS represented no statistical significance). E Interaction between endogenous TPI1 and CDCA5. IP lysates were used to analyze expression of TPI1 and CDCA5 by western blot. F Immunofluorescence used to verify formation of TPI1/CDCA5 complex in those indicated cells, Scale bar, 10 μm. G T47D cells stably expressing control, sh2 and sh3 were treated with CHX (200 μg/ml) for indicated time points (0, 2, 4, 6, 8 h, respectively). Cells were lysed and examined by western blot. H MDA-MB-231 cells expressing vector and TPI1 were treated with CHX (200 μg/ml) for indicated time points (0, 2, 4, 6, 8, 10, 12 h, respectively). Cells were lysed and examined by western blot. β-Actin served as a control. The numbers represented relative expression of CDCA5 in G and H. NS represented no statistical significance

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