Fig. 1

High expression of TPI1 positively correlates with poor prognosis in BRCA. A Expression of glycolysis and gluconeogenesis related genes in normal and tumor breast tissues. B Univariate Cox regression model was applied to calculate hazard ratios (HRs) of glycolysis-related genes from Tang (2018) dataset of Kaplan–Meier plotter database. C TPI1 gene expression in human BRCA tissues and matched normal tissues (n = 112) from TCGA. D, E Expression of TPI1 in BRCA based on individual clinical stages and lymph node stages (N) (TCGA). F TPI1 expression levels in fresh BRCA tumor (T) tissues and matched normal (N) tissues were examined by western blotting. G Representative image of IHC staining of TPI1 in BRCA tissue microarray. Magnification, ×40 and ×400. H TPI1 expression levels were compared in BRCA tumor tissue and normal tissue. I, J Kaplan–Meier curves to display relationships between TPI1 expression and DFS or OS in BRCA patients in Harbin Medical University Cancer Center (HMUCC) (I) and METABRIC database (J), respectively. K Western blotting analysis of TPI1 expression in MCF-10A cells and eight human BRCA cell lines. β-Actin served as a control. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)