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Fig.3 | Journal of Translational Medicine

Fig.3

From: Ruxolitinib suppresses liver fibrosis progression and accelerates fibrosis reversal via selectively targeting Janus kinase 1/2

Fig.3

JAK1 and JAK2 antagonism had anti-fibrotic activity in vitro. A The protein levels of JAK downstream in LX-2 cells treated with different concentrations of Ruxolitinib. B Ruxolitinib inhibited cell proliferative effect in LX-2 cells in a dose and time-dependent manner. C The half-maximal inhibitory concentration (IC50) value of Ruxolitinib on LX-2 cells. D The upper panel shows relative mRNA expression of ACTA2, PDGFRβ, COL1A1, and PAI-1 in LX-2 cells treated with different concentrations of Ruxolitinib. The below shows protein expression of α-SMA, PDGFRβ, and PAI-1. E The left panel shows that treatment with Ruxolitinib inhibited LX-2 cell proliferation (× 100 magnification). The right panel shows the number of migration counted in LX-2 cells treated with different concentrations of Ruxolitinib. F The left panel shows that treatment with Ruxolitinib promoted LX-2 cells apoptosis. The right panel shows the apoptosis rate of LX-2 cells treated with different concentrations of Ruxolitinib. Data presented are means ± SD. NS not significant; *P < 0.05; **P < 0.01; ***P < 0.001

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