Fig.2

Knockdown of JAK1 and JAK2 inhibits activation, proliferation and migration of HSCs. A The left panel shows LX-2 cells co-interfered with siJAK1 and siJAK2 reduced JAK1 and JAK2 mRNA expression. The right panel shows that siJAK2 reduced JAK1 and JAK2 protein expression in LX-2 cells co-interfered with siJAK1. B The left panel shows that knockdown JAK1 and JAK2 reduced mRNA expression of ACTA2, PDGFRβ in LX-2 cells. The right panel shows knockdown JAK1 and JAK2 reduced protein expression of α-SMA, PDGFRβ. C Knockdown expression of JAK1 and JAK2 inhibited LX-2 cell proliferation. D The left panel shows knockdown JAK1 and JAK2 inhibited migration (× 100 magnification). The right shows the number of migrations counted after interfered with JAK1 and JAK2 in LX-2 cells. E LX-2 cells knockdown JAK1 and JAK2 reduced protein expression of p-JAK1 (Tyr1022/1023), p-JAK2 (Tyr1007/1008), STAT3, p-STAT3 (Tyr705), p-STAT3 (Ser727). Date presented are means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001