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Fig. 8 | Journal of Translational Medicine

Fig. 8

From: The role of neutrophil elastase in aortic valve calcification

Fig. 8

Alvelestat ameliorates valve thickness and partly improves cardiac functions of APOE−/− mice. A HE and immunohistochemical staining analysis of NE and α-SMA expression of aortic valve in APOE−/− mice. For normal diet group (ND), n = 5. For normal diet + Alvelestat group (ND + Alv), n = 4. For western diet group (WD), n = 3. For western diet + Alvelestat group (WD + Alv), n = 5. Scar bar = 100 µm, Scar bar = 50 µm. B Quantitative analysis of valve thickness in each group. C–E cusp separation, transaortic peak velocity and ejection fraction (EF) were measured by echocardiography in each group. F NE activity in each group was measured by N-methoxysuccinyl-Ala-Ala-pro-Val-p-nitroanilide. This experiment weas independently repeated three times. *P < 0.05, ***P < 0.001, ****P < 0.0001 vs corresponding control groups. The measurement data are expressed as the mean ± SD

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