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Fig. 7 | Journal of Translational Medicine

Fig. 7

From: The role of neutrophil elastase in aortic valve calcification

Fig. 7

Intracellular signal pathways involved in NE effects. A–E pVICs were treated with NE for 24 h in normal medium or in osteogenic medium. Western blot analysis of Smad 1/5/8, phosphorylated Smad 1/5/8 (p-Smad 1/5/8), ERK, phosphorylated ERK (p-ERK), P65, phosphorylated P65 (p-P65), AKT and phosphorylated AKT(p-AKT). F–J Western blot analysis of Smad 1/5/8, p-Smad 1/5/8, ERK, p-ERK, P65, p-P65, AKT and p-AKT. K, L pVICs were treated with 10 nmol/L LY294002 and BAY11-7082 1 h before the addition of NE. The expression of OPN was measured by Western blot. All experiments were performed at least in triplicate. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs corresponding control groups

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