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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: The role of neutrophil elastase in aortic valve calcification

Fig. 1

The expression and activity of NE in the serum and calcified aortic valve from patients with CAVD. A Detection of the circulating levels of NE in patients with CAVD and non-CAVD by ELISA. B NE activity in patients with CAVD and non-CAVD was measured by N-methoxysuccinyl-Ala-Ala-pro-Val-p-nitroanilide. For non-CAVD patients, n = 30. For CAVD patients, n = 58. *P < 0.05, **P < 0.01 vs non-CAVD group. C Western blot analysis of RUNX2, OPN and NE protein expression in the normal AV and calcified AV. *P < 0.05, **P < 0.01 vs normal AV. D Representative micrographs of aortic valve sections from patients with CAVD and non-CAVD for HE, ARS, OPN and NE. The black arrow indicates the positive area. Scar bar = 500 µm, Scar bar = 50 µm. E, F WB analysis of NE expression and G detection of NE activity in pVICs cultured in osteogenic medium for 24 h. **P < 0.01 vs Blank group. The measurement data are expressed as the mean ± SD. All experiments were independently repeated three times

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