Fig. 3
From: Phosphorylated ATF1 at Thr184 promotes metastasis and regulates MMP2 expression in gastric cancer
The expression and activity of MMP2 is up-regulated by P-ATF1-T184. A Real-time quantitative RT-PCR detection of ATF1 and MMP2. B The correlation analysis between the mRNA expression of ATF1 and MMP2. C Western blot showed the expression of phosphorylation ATF1-T184 and protein expression of ATF1, MMP2 in different cell lines. The quantitative data of p-ATF1-T184 protein were presented in the right panel. D Effect of ATF1-T184D on mRNA level of MMP2 in AGS cells. E Effect of ATF1-T184A on mRNA level of MMP2 in MGC803 cells. F Western blot showed the effect of ATF1-T184D and ATF1-T184A on the protein expression of MMP2. G Gelatinase assay revealed the effect of ATF1-T184D and ATF1-T184A on the activity of MMP2 in gastric cancer cells MGC803. Experiments were carried out using transient transfection with corresponding plasmid