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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: The SETD8/ELK1/bach1 complex regulates hyperglycaemia-mediated EndMT in diabetic nephropathy

Fig. 6

The transcriptional activity of bach1 is jointly regulated by ELK1 and SETD8 in HGECs. a ELK1 and H4K20me1 accumulated at the bach1 promoter region. b Schematic diagram of the bach1 promoter region containing an E-box and the conserved ELK1 binding site. c, d Bach1 promoter activity was determined by luciferase reporter assays after treatment. e ELK1 and SETD8, as well as K4K20me1, were located on the same promoter region of bach1 in HGECs. f The binding of ELK1 to the promoter region of bach1 was regulated by SETD8 in HGECs. g Western blot results of genes in different cell groups. h, i mRNA expression of genes in different cell groups (n = 5/group). j Western blot results of genes in different cell groups. k, l mRNA expression of genes in different cell groups (n = 5/group). m Western blot results of genes in different cell groups. n, o mRNA expression of genes in different cell groups (n = 5/group, data are presented as the mean ± standard deviation, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, statistical analysis was carried out by one-way ANOVA)

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