Fig. 4

ELK1 is related to SETD8. a Co-IP verified the connection between ELK1 and SETD8 in HGECs. b GST pulldown verified the direct connection between ELK1 and SETD8 in HGECs. c Immunofluorescence assays illustrated the nuclear translocation of ELK1 under HG environment. Further, the result verified the colocalization of ELK1 and SETD8 in HGECs (scale bar: 50 μm). d Western blot results of genes in different cell groups. e mRNA expression of genes in different cell groups (n = 5/group, data are presented as the mean ± standard deviation, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, statistical analysis was carried out by one-way ANOVA)