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Table 1 Compound effects of viable cell recovery and impaired lytic activity on NK-92 cell functionality one day after irradiation*

From: Optimizing NK-92 serial killers: gamma irradiation, CD95/Fas-ligation, and NK or LAK attack limit cytotoxic efficacy

Irradiation (Grays) % Control viable cell recovery+ Serial KF % Control KF % Remaining functional activity based on KF# LU50 per 106
NK-92 cells
% Control lytic units % Remaining functional activity based on lytic units^
0 100 5.6 100 100 815.5 100 100
2.5 53.4 3.9 69.6 37.2 419.8 51.5 27.5
5 56.8 2.5 44.6 25.4 215.9 26.5 15.0
10 39.0 1.5 26.8 10.4 23.8 2.9 1.1
  1. *Raji 'target' cells at E:T 1:16 after 8 h. This representative experiment was comparable to two other replicate experiments
  2. +Cells proliferated to increase 75–80% from time of initial culture without irradiation
  3. #Calculated as the fraction representing viable cell recovery multiplied by the fraction of control KF
  4. ^Calculated as the fraction representing viable cell recovery multiplied by the fraction of lytic unit activity that was retained compared to untreated NK-92 cells