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Table 1 Compound effects of viable cell recovery and impaired lytic activity on NK-92 cell functionality one day after irradiation*

From: Optimizing NK-92 serial killers: gamma irradiation, CD95/Fas-ligation, and NK or LAK attack limit cytotoxic efficacy

Irradiation (Grays)

% Control viable cell recovery+

Serial KF

% Control KF

% Remaining functional activity based on KF#

LU50 per 106

NK-92 cells

% Control lytic units

% Remaining functional activity based on lytic units^

0

100

5.6

100

100

815.5

100

100

2.5

53.4

3.9

69.6

37.2

419.8

51.5

27.5

5

56.8

2.5

44.6

25.4

215.9

26.5

15.0

10

39.0

1.5

26.8

10.4

23.8

2.9

1.1

  1. *Raji 'target' cells at E:T 1:16 after 8 h. This representative experiment was comparable to two other replicate experiments
  2. +Cells proliferated to increase 75–80% from time of initial culture without irradiation
  3. #Calculated as the fraction representing viable cell recovery multiplied by the fraction of control KF
  4. ^Calculated as the fraction representing viable cell recovery multiplied by the fraction of lytic unit activity that was retained compared to untreated NK-92 cells