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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Optimizing NK-92 serial killers: gamma irradiation, CD95/Fas-ligation, and NK or LAK attack limit cytotoxic efficacy

Fig. 5

Killing of NK-92 cells by either resting or activated primary blood NK cells. The susceptibility of NK-92 tumor cells to attack by primary peripheral blood NK cells from healthy donors was investigated using NK-92 cells that were either non-irradiated or were irradiated with 10 Gy and then cultured for 1 day. The primary NK cells were either resting or activated by 3 days culture with 1000 u/ml IL-2. Cytotoxicity was measured using 4-h 51Cr-release assays; the gray dashed line reflects the 10% threshold for positive killing. The results illustrated represent concurrent assays made with E:Ts of viable effector NK cells from a single donor SC-0975 and are representative of 4 experiments each with a different donor. A Killing of NK-92 cells by resting primary NK cells. LU50s were excluded for unstimulated NK cells because killing was over 10% for only the highest E:T. B Killing of NK-92 cells by activated primary NK cells. For both bNKs and LAKs, the cytotoxicity towards non-irradiated versus irradiated NK-92 cells was significantly different at the highest E:T (student t-test, *p < 0.03, **p < 0.005). Insert for B LU50/1 M LAK bNK calculated using TruCOUNT™ beads (Gating—Additional file 2: Fig. S1)

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