Fig. 5

CircZDBF2 regulates RNF145 by recruiting CEBPB. A Luciferase reporter assay was taken to analyze the binding situation of circZDBF2 to RNF145 promoter. B UCSC database was applied to predict the possible transcription factors for circZDBF2. C The possibility of circZDBF2 combining with CEBPB was predicted through RPISeq database. D Luciferase reporter assay was applied to detect the binding of CEBPB to RNF145 promoter. E The enrichment of RNF145 promoter in anti-IgG group and anti-CEBPB group was measured by ChIP assay. F Luciferase reporter assay was taken to verify the specific sites that CEBPB bound to RNF145 promoter. G The enrichment of CEBPB with or without the biotinylated circZDBF2 probe was measured by RNA pull down assay. H CircZDBF2 enrichment in anti-IgG group and anti-CEBPB group was analyzed by RIP assay. I The relative enrichment of RNF145 promoter in anti-IgG group and anti-CEBPB group was measured by ChIP assay upon circZDBF2 downregulation. J RNF145 expression was detected by qRT-PCR in different groups. ^**P < 0.01