Fig. 4

RNF145 is the target gene of miR-362-5p and miR-500b-5p. A, B Possible mRNAs of miR-362-5p and miR-500b-5p were forecast through ENCORI database. C RNF145, SAMD4A and ATP2B4 expression was detected by qRT-PCR after circZDBF2 was knocked down in OSCC cells. D The expression of RNF145 was detected by qRT-PCR in OSCC cells transfected with miR-362-5p mimics or miR-500b-5p mimics. E–G RNA pull down assay, together with luciferase reporter assay was performed to prove the binding of RNF145 with miR-362-5p or miR-500b-5p. H RNF145 expression was detected via qRT-PCR in different groups transfected with sh-NC, sh-circZDBF2-1, sh-circZDBF2-1 + inhibitor-NC, sh-circZDBF2-1 + miR-362-5p inhibitor and sh-circZDBF2-1 + miR-362-5p inhibitor + miR-500b-5p inhibitor plasmids. ^*P < 0.05, ^**P < 0.01