Fig. 4

TEC inhibited pyroptosis by promoting autophagy in NASH cells. A The expression of LC3B in hepatocytes was detected by immunofluorescence. Scale bar = 50 μm. B The Oil red O staining demonstrated that TEC reduced lipid deposition through autophagy activation. Scale bar = 50 μm. C The NLRP4 and GSDME protein expression were detected by western blot assay. D The release of LDH. **P<0.01, ***P<0.001 (n=3). 3MA: autophagy inhibitor