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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Computer-assisted three-dimensional quantitation of programmed death-ligand 1 in non-small cell lung cancer using tissue clearing technology

Fig. 4

Application of 3D PD-L1 scoring method to actual non-small cell lung cancer needle biopsy specimen shows difference compared to originally reported TPS and may explain unexpected immunotherapy treatment responses. A Two lung cancer patients with 0% tumor PD-L1 expression as evaluated by the standard immunohistochemistry method achieved stable disease or partial response under nivolumab treatment. BD 3D PD-L1 expression analysis of case 34 showed higher tumor cell PD-L1 expression at deeper levels, reaching 5.67%, above the 1% cut-off value. EG Analysis of case 35 also showed higher tumor cell PD-L1 expression at deeper levels, up to 17.28% (SD: stable disease; PR: partial response; IF: immunofluorescence. Green: PD-L1 and blue: SYTO-16. Scale bar: 50 μm)

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