Fig. 6

The circESRP1/miR-874-3p/CPEB4 axis promoted EC progression by activating EMT. a The Transwell assay indicated that the migration and invasion abilities of Ishikawa cells and RL952 cells transfected with the circESRP1 vector were counteracted when the cells were cotransfected with the miR-874-3p mimic. b Western blot analysis showed that the levels of EMT-related proteins and CPEB4 transfected with the circESRP1 vector were rescued when Ishikawa cells and RL952 cells were cotransfected with the miR-874-3p mimic. c The results of Transwell assays indicated that sh-CPEB4 inhibited the promotive effect of circESRP1 on Ishikawa cells and RL952 cell metastasis. d Western blot analysis showed that the level of EMT-related proteins transfected with circESRP1 vector was rescued when Ishikawa cells and RL952 cells were cotransfected with sh-CPEB4. Scale bar: 100 μm, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001