Fig. 1

Interactions between TNBC and tumor cells result in upregulation of inflammatory cytokines in tumor cells. a Real-time PCR analysis of SUM159 tumor cells co-cultured with B cells showing increases in inflammatory cytokines over non-co-cultured cells. b SUM159 tumor cells or MDA-MB-231 cells after 24 h co-culture with B cells result in an increased gene expression of IL-10 and IL-4. Graph shows fold change of co-cultured cells compared to controls. Error bars represent standard deviations. These experiments were completed in at least triplicate. c Elisa showing increased protein expression (pg/ml) of IL-10 in SUM159 and MDA-MB-231 cells after 24 h co-culture with B cells. This experiment was performed in duplicate in each cell line. Error bars represent standard deviation of technical replicants. Significance is indicated by asterisk, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.01, ns p > 0.05