Fig. 4

Mixed gastric adenocarcinoma-lymphoma PDXs can be “rescued” by propagation in nude mice. The PDX was initially established in NSG mice and contained both gastric adenocarcinoma and B-cell lymphoma as shown by A H&E, B pan-cytokeratin IHC (pink arrowheads to positive staining gastric adenocarcinoma cells, green arrows to negative staining lymphoma cells), and C CD20 IHC (pink arrowheads to negative staining gastric adenocarcinoma cells, green arrowheads to positive staining lymphoma cells). After transplantation to nude mice, the tumor contained only gastric adenocarcinoma as confirmed by D H&E, E pan-cytokeratin IHC, and F CD20 IHC (yellow arrowheads show non-specific staining in glandular lumens)