Fig. 7

NAMPT possesses oncogenic properties. A Basal cell growth of NAMPT over-expressing (NAMPT) vs. control GFP (CTRL) cells, as measured by the MTT assay. Data are from 3 independent experiments (each performed in triplicate) and are represented as absorbance 595 nm optical density (O.D.). B Colony-forming ability (colony-forming units CFU) of Mewo and NIH-3T3 NAMPT over-expressing in comparison with control cells for 12 days. Cells were stained with crystal violet and representative images are shown. On the right, histograms show the cumulative quantification of the percentage (%) area with colonies at the end of the 12-days period (3 independent experiments performed in triplicates). Data in the Figure are presented as the mean ± SEM. Statistical significance was calculated using Mann–Whitney test. Significance was represented as: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001