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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Activation of miR-500a-3p/CDK6 axis suppresses aerobic glycolysis and colorectal cancer progression

Fig. 5

CDK6 is upregulated and promotes aerobic glycolysis in CRC. A, B The abundance of CDK6 was examined in CRC tissue samples (n = 20) and adjacent normal tissue samples (n = 20) by IHC staining. Scale bar, 100 μm. Representative images (left) and quantification (right) were shown. C–E The glucose uptake (C), lactate production (D), intracellular ATP (E) were measured in SW480 cells after transfected with vector and CDK6. F The extracellular acidification rate (ECAR) of SW480 cells were analyzed by Seahorse XF96 Extracellular Flux Analyzer after transfected with vector and CDK6. Data were shown as the mean ± SD of three independent experiments. G, H The mRNA level of GLUT1, LDHB, HK1 and HK2 (G) by qPCR and the protein level of GLUT1 and HK2 (H) by western blot in SW480 cells transfected with vector and CDK6. I Spearman correlation between IHC score of CDK6 and HK2 were conducted in the cohort (n = 134). *P < 0.05, **P < 0.01, ***P < 0.001

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