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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Activation of miR-500a-3p/CDK6 axis suppresses aerobic glycolysis and colorectal cancer progression

Fig. 3

miR-500a-3p suppresses aerobic glycolysis in CRC. A–C The glucose uptake (A), lactate production (B), intracellular ATP (C) were measured in HCT116 and SW480 cells after cultured with FBS-free medium for 24 h using Glucose Uptake Colorimetric Assay kit, Lactate Assay Kit and ATP Colorimetric Assay kit. D The extracellular acidification rate (ECAR) of SW480 cells were analyzed by Seahorse XF96 Extracellular Flux Analyzer. The data from A to D were presented as the means ± SD (n = 3). E Heatmap showed the level of 29 metabolites related to energy metabolism in SW480 cells transfected with miR-NC and miR-500a-3p. 5 glycolytic metabolites whose levels were significantly different between two groups were marked in the red box. F, G The mRNA level of HK2, PFKP, PGK1 and LDHA (F) by qPCR and the protein level of GLUT1, HK2 and PKM2 (G) by western blot in SW480 cells transfected with miR-NC and miR-500a-3p. β-Actin served as control. H The specimens in the cohort which contained 134 continuous CRC patients were stained with HK2. Scale bar, 100 μm. I Association between miR-500a-3p mRNA level and HK2 IHC score were conducted in the cohort (n = 134). (Spearman’s correlation analysis) *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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