Fig. 7

USP3 mediates SNAIL1 deubiquitination. A The protein which can interact with USP3 was predicted by BioGrid database (https://thebiogrid.org/) under the condition of Evidence ≥ 3. B The protein levels of the candidates was analyzed after SND1-IT1 down-regulation in GES-1 cells. C USP3 and SNAIL1 combination was verified by Co-IP assay. D, E In GES-1 cells, SNAIL1 RNA and protein levels after USP3 knockdown were detected by q-PCR and western blot respectively. F SNAIL1 ubiquitination level under different conditions was detected by IP-western blot assay. G. SNAIL1 protein level was detected by western blot assay after the transfection of si-NC, si-SND1-IT1-1, si-SND1-IT1-1 + pcDNA3.1 or si-SND1-IT1-1 + pcDNA3.1-USP3. H. Western blot assay was utilized to measure the SNAIL1 protein level under different time points after addition with CHX