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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Exosome-mediated lncRNA SND1-IT1 from gastric cancer cells enhances malignant transformation of gastric mucosa cells via up-regulating SNAIL1

Fig. 5

SND1-IT1 recruits DDX54 to facilitate USP3 mRNA stability. A The Venn diagram showed the RBPs (TARDBP, DDX54 and HNRNPC) binding to both SND1-IT1 and USP3, which are predicted by starBase. B In GES-1 cells, the interaction of SND1-IT1 with RBP candidates was detected by RIP assay. C RNA pull-down assay detected the interaction of SND1-IT1 with DDX54. D DDX54 was predicted to bind to USP3 3′UTR by using starBase. E In GES-1 cells, western blot was used to verify the binding between USP3 3′UTR and DDX54 after RNA pull-down assay. F In GES-1 cells, RIP assay was used to verify the binding between USP3 3′UTR and DDX54 after. G The expression of DDX54 in GES-1 cells was measured by q-PCR after the overexpression of SND1-IT1. H In GES-1 cells, the binding between USP3 3′UTR and DDX54 was detected by RIP assay before and after SND1-IT1 overexpression. I USP3 and β-actin half-lives were measured by q-PCR after DDX54 was interfered. **P < 0.01

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