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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Exosome-mediated lncRNA SND1-IT1 from gastric cancer cells enhances malignant transformation of gastric mucosa cells via up-regulating SNAIL1

Fig. 4

SND1-IT1 competitively adsorbs miR-1245b-5p to up-regulate USP3 expression. A SND1-IT1 enrichment in Anti-AGO2 group was detected by RIP assay in GES-1 cells. B The miRNAs which can bind to SND1-IT1 were predicted by starBase (https://rna.sysu.edu.cn/encori/index.php) when AgoExpNum ≥ 2 and Pan-Cancer ≥ 3. C In GES-1 cells, the interaction of SND1-IT1 with miR-873-3p and miR-1245b-5p was detected by RNA pull-down assay. D The interaction between SND1-IT1 and miR-1245b-5p was detected by RNA pull-down assay. E The expression of miR-1245b-5p was detected by q-PCR after SND1-IT1 was overexpressed. F In 293 T cells, dual-luciferase reporter assay detected the interaction between SND1-IT1 and miR-1245b-5p. G The mRNA which can bind to miR-1245b-5p was predicted and screened by starBase database. H In GES-1 cells, q-PCR detected the expression of candidate mRNAs before and after up-regulation of SND1-IT1. I In 293 T cells, dual-luciferase reporter assay detected the interaction between USP 3′UTR and miR-1245b-5p. J The expression level of USP3 was detected by q-PCR in GES-1 cells after the transfection of pcDNA3.1, pcDNA3.1-SND1-IT1, pcDNA3.1-SND1-IT1 + mimics NC or pcDNA3.1-SND1-IT1 + miR-1245b-5p mimics. K The interaction between USP3 and miR-1245b-5p was detected in GES-1 cells by RNA pull-down assay before and after SND1-IT1 overexpression. L In 293 T cells, dual-luciferase reporter assay was performed to detect the interaction between USP3 3′UTR and miR-1245b-5p before and after SND1-IT1 overexpression. **P < 0.01

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