Fig. 2

GC cells secrete SND1-IT1 via exosomes. A The list of lncRNAs significantly overexpressed in plasma exosomes of GC patients was screened by GSE153413 dataset under the condition of p < 0.05 and logFC > 2. B NTA detection was taken to examine the diameters of Exo/AGS and Exo/GES-1. C The morphology of exosomes was observed by an electron microscope. D Western blot was performed to detect the expression of CD63, CD81 and CD9. E. SND1-IT1, VWA8-AS1 and LINC00511 expressions were measured by q-PCR in Exo/AGS and Exo/GES-1. F. The expression of SND-IT1 in the plasma exosomes of GC patients was analyzed through GSE153414. G NTA detection was taken to examine the diameters of Exo/pcDNA3.1 and Exo/SND1-IT1. H The morphology of Exo/pcDNA3.1 and Exo/SND1-IT1 was observed by electron microscope. I Western blot was conducted to detect the protein levels of CD63, CD81 and CD9 in Exo/pcDNA3.1 and Exo/SND1-IT1. J The expression of SND1-IT1 in Exo/pcDNA3.1 and Exo/SND1-IT1 was detected by q-PCR. K GES-1 cells were treated with Exo/pcDNA3.1 and fluorescent-labeled Exo/SND1-IT1 respectively, and the cell fluorescence was observed under a fluorescence microscope. ^**P < 0.01