Fig. 3

Down-regulated miR-200c promoted growth and metastasis in bladder cancer cells. A Antagonizing endogenous miR-200c in UMUC-3 and T24 cells. Level of miR-200c was measured by real-time PCR and miR-200c was obviously antagonized in the both cell lines. B CCK-8 assays revealed cell proliferation differences of T24 and UMUC-3 cells. C Colony formation assay in indicated cell lines. D Measurement of in vitro cell migration by wound-healing assay. E Transwell migration assays in T24 and UMUC-3 cells. F Transwell invasion cells in T24 and UMUC-3 cells. Data are presented as mean ± SD from 3 independent experiments. *P < 0.05; **P < 0.01. ***P < 0.001; DAPI, 4′,6-diamidino-2-phenylindole