Fig. 2

Stimulation of NK cells by 50 µM PregS following overnight stimulation with IL-2 and treatment with NTX. A Proportional mean data from Ca²⁺ imaging experiments comparing normalised slope of 50 µM PregS Ca²⁺ influx curve. B Proportional mean data from Ca²⁺ imaging experiments comparing normalised T1/2 (seconds) response of maximum 50 µM PregS Ca²⁺ response. C Proportional mean data from Ca²⁺ imaging experiments comparing normalised amplitude (nm) of maximum 50 µM PregS Ca²⁺ response. D Example time-course responses to 50 µM PregS and 1 µM Ionomycin in HC. E Example time-course responses to 50 µM PregS and 1 µM Ionomycin in ME/CFS patients. Data was collected from n = 10 HC and n = 10 ME/CFS patients. All Ca²⁺ influx measurements for PregS were normalised using Ionomycin response curves. Total number of cells following the removal of outliers are included within bar graphs. Data presented as mean ± SD. HC healthy controls, ME myalgic encephalomyelitis, CFS chronic fatigue syndrome, T1/2 half-time, nm nanometres, PregS, pregnenolone sulfate, SD standard deviation, ns no significance