Fig. 4

The effects of UAP1L1 overexpression and CDCA8 knockdown on prostate cancer cells were demonstrated in vitro. A MTT assay results indicated the influence of UAP1L1 overexpression and CDCA8 knockdown on the proliferation of prostate cancer cells. B The migration ability of C4-2 cells was determined by wound healing assay and the migration rate was increased by upregulating UAP1L1, but reduced after CDCA8 knockdown. C Transwell assay revealed that UAP1L1 overexpression promoted the invasion ability of prostate cancer cells, which could be inhibited by CDCA8 knockdown. D Flow cytometry was performed for detecting the apoptosis of C4-2 cells. Control, shCtrl and NC: negative control; UAP1L1: prostate cancer cells overexpressed UAP1L1; shCDCA8: prostate cancer cells infected with shRNA of CDCA8; UAP1L1 + shCDCA8: prostate cancer cells overexpressed UAP1L1 as well as infected with shRNA of CDCA8. *P < 0.05; ***P < 0.001