Fig. 6

miR-889-3p reverses the inhibitory effect of circDYRK1A on malignant phenotypes of GC cells. AGS cells were transduced with miR-889-3p mimic and/or circDYRK1A, and HGC-27 cells were transduced with miR-889-3p inhibitor and/or sh-circDYRK1A. A Expression of circDYRK1A and miR-889-3p in AGS and HGC-27 cells determined by RT-qPCR. B Proliferation of AGS and HGC-27 cells detected by EdU assay. C Migration of AGS and HGC-27 cells detected by Transwell assay. D Invasion of AGS and HGC-27 cells detected by Transwell assay. E Expression of glutamine in AGS and HGC-27 cells measured using the kit. F Expression of glutamic acid in AGS and HGC-27 cells measured using the kit. G Expression of α-KG in AGS and HGC-27 cells measured using the kit. H Protein levels of GLS and GDH in AGS and HGC-27 cells measured by Western blot analysis. The cell experiment was repeated three times. * p < 0.05