Fig. 3

CircDYRK1A inhibits glutamine metabolism in GC cells. AGS cells were transduced with circDYRK1A overexpression vector and HGC-27 cells were transduced with sh-circDYRK1A. A The expression of glutamine in AGS and HGC-27 cells detected using the kit. B The expression of glutamic acid in AGS and HGC-27 cells detected using the kit. C The expression of α-KG in AGS and HGC-27 cells detected using the kit. D The protein levels of GLS and GDH in AGS and HGC-27 cells detected by Western blot analysis. The cell experiment was repeated three times. * p < 0.05 vs. sh-NC-treated HGC-27 cells, or Vector-treated AGS cells