Fig. 1

CircDYRK1A is poorly expressed in GC tissues and cells. A Unsupervised heat map of differentially expressed miRNAs in microarray dataset GSE93541. B Volcano map of differentially expressed miRNAs in microarray dataset GSE93541. C Unsupervised thermogram of differentially expressed miRNAs in microarray dataset GSE89143. D The volcano map of differentially expressed miRNAs in microarray dataset GSE89143. E Venn diagram of downregulated circRNAs in microarray datasets GSE93541 and GSE89143. F The expression of hsa_circRNA_001826 (circDYRK1A) in microarray dataset GSE9354. G The expression of hsa_circRNA_001826 (circDYRK1A) in microarray dataset GSE89143. H Expression of circDYRK1A in GC tissues and adjacent normal tissues (n = 50) determined RT-qPCR. I Expression of circDYRK1A in GC tissues and adjacent normal tissues detected by ISH assay. J Expression of circDYRK1A in normal gastric mucosa cell line GES-1 and GC cell lines AGS, MKN74, HGC-27, N87, SNU-5 and HS-746 T determined RT-qPCR. K The splicing form of circDYRK1A in AGS cells and HGC-27 cells detected by gel electrophoresis. indicates the amplification results of divergent primers and indicates the amplification results of convergent primers. L Expression of circDYRK1A and DYRK1A in AGS cells and HGC-27 cells in the presence or absence of RNase R measured by RT-qPCR. M The location of circDYRK1A in AGS and HGC-27 cells detected by FISH assay. N Expression of circDYRK1A in patients with early GC (I + II) (n = 18) and patients with advanced GC (III + IV) (n = 32) detected by RT-qPCR. O The overall survival (OS) and disease-free survival (DFS) time of patients with GC analyzed by Kaplan–Meier analysis. The cell experiment was repeated three times. * p < 0.05 vs. adjacent normal tissues, GES-1 cells or Mock, or stage I + II