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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: CRISPR screening of E3 ubiquitin ligases reveals Ring Finger Protein 185 as a novel tumor suppressor in glioblastoma repressed by promoter hypermethylation and miR-587

Fig. 6

MiR-587 regulates RNF185 expression and promotes glioma cell proliferation and migration. A The intersection analysis of predicted miRNAs by three online tools; B correlation analysis of RNF185 with miR-525 and miR-587; C CCK8 assays showing the cell number of miR-587 inhibitor treated glioma cells; D Annexin V/PI staining demonstrates the function of miR-587 inhibitor on glioma cells apoptosis; E wound healing assay shows the function of miR-587 inhibitor on glioma cells migration; F the expression of RNF185 in miR-587 inhibitor treated glioma cells; G dual luciferase assay showing the binding site and regulatory effect of miR-587 on RNF185 mRNA; All experiments were conducted for three replicates; *p < 0.05, **p < 0.01

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