Fig. 1

CRISPR screening of E3 ligases reveals significantly changed genes (SCGs) in glioma cell line U87. A The workflow chat of CRISPR screening assays of E3 ligases in glioma U87 cells; B volcano plot showing the significantly enriched and lost genes; enriched genes were shown with red dots, and lost genes were shown with green dots; C reads counts in the first and 4th generation cells; D protein domain analysis of enriched and lost genes