Fig. 3

Linc00261 regulates CSCs related markers’ expression in HCC cells. A, B Western blotting analysis (A) and immunofluorescence staining analysis (B) of CSC-markers (CD44 and CD133) and -transcription factors (SOX2 and OCT4) in SMMC-7721 cells after overexpression of linc00261 using lentivirus. The histogram represents quantitative analyses of the relative gray values of the western blotting bands. C Western blotting analysis of CSC-markers (CD44 and CD133) and CSC-transcription factors (SOX2 and OCT4) protein levels in Huh7 cells and MHCC-LM3 cells after transfection with control siRNA or siRNAs against linc00261. The histogram represents quantitative analyses of the relative gray values of western blotting bands. D Linc00261 overexpression inhibits sphere formation both numbers and size in SMMC-7721 cells.* P < 0.05; ** P < 0.01; *** P < 0.001