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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: TGF-β1 induced deficiency of linc00261 promotes epithelial–mesenchymal-transition and stemness of hepatocellular carcinoma via modulating SMAD3

Fig. 2

Linc00261 inhibits EMT in HCC cells. A Quantitative RT-PCR analysis of linc00261 expression in HCC cells after linc00261 overexpression (SMMC-7721, Sk-hep1) and linc00261 knockdown (Huh7, MHCC-LM3) using lentivirus and specific small interfering RNAs (siRNAs), respectively. B The protein expressions of epithelial (E-cadherin) and mesenchymal associated marker (Vimentin)/transcription factors (ZEB1 and Slug) were determined by western blotting after linc00261 overexpression in SMMC-7721 and Sk-hep1, or linc00261 knockdown in Huh7 and MHCC-LM3 cells. The gray value of these proteins were evaluated by normalizing to β-actin. C Linc00261 overexpression promotes the morphological transition from mesenchymal to epithelial states in SMMC-7721 and SK-Hep1. * P < 0.05; ** P < 0.01; *** P < 0.001

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Journal of Translational Medicine

ISSN: 1479-5876

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