Fig. 1
TGF-β1 induced the dysregulation of linc00261 and EMT/CSCs associated proteins in HCC cells. Linc00261 was down-regulated after TGF-β1 treatment in Huh7 (A) and HepG2 (B) cells determined by qRT-PCR. qRT-PCR analysis of linc00261 expression after treating with TGF-β1 and SB431542, a TGF-β1 inhibitor in Huh7 (C) and HepG2 (D) cell lines. E qRT-PCR analysis of linc00261 expression at time points of 0, 1, 2, 3, 4, and 5 h in Huh 7 cells after TGF-β1 treatment with 5 ng/ml. F Western blotting analysis of EMT, CSCs and TGF-β1 pathway related proteins after TGF-β1 treatment. G The relative gray value (normalized to β-actin) evaluations of the EMT, CSCs and TGF-β1 pathway related proteins in E.* P < 0.05; ** P < 0.01; *** P < 0.001