Fig. 1

NEAT1 promotes cell proliferation and glycolysis. A Relative NEAT1 expression levels in U251 and T98G cells transfected with sh-ctrl or sh-NEAT1. B CCK-8 analysis of U251 and T98G cells transfected with sh-ctrl or sh-NEAT1. C Colony formation assays of U251 and T98G cells transfected with sh-ctrl or sh-NEAT1. D Western blot analysis of CDK2, CDK4, and CDK6 in U251 and T98G cells transfected with sh-ctrl or sh-NEAT1. E, F ECAR was measured through the Glycolysis Stress in U251 cells transfected with sh-ctrl or sh-NEAT1. G, H ECAR was measured through the Glycolysis Stress in T98G cells transfected with sh-ctrl or sh-NEAT1. I Lactate concentration was assessed in U251 and T98G cells transfected with sh-ctrl or sh-NEAT1. *p < 0.05, **p < 0.01. Error bars indicate mean ± SD of triple independent experiments