Skip to main content
Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Membrane progesterone receptor α (mPRα) enhances hypoxia-induced vascular endothelial growth factor secretion and angiogenesis in lung adenocarcinoma through STAT3 signaling

Fig. 6

mPRα enhances HUVEC tube formation and migration under hypoxia through HIF1α-induced VEGF release, and STAT3 signaling A549 cells were transfected with si-mPRα or si-NC (negative control). The culture medium was collected for HUVEC culture (marked as si-mPRα-CM/si-NC-CM). HUVECs were cultured in different conditioned mediums under 1% O2 and examined for A cell migration by wound healing assay, B tube formation by Tube formation assay, and C VEGF content in the culture medium by ELISA. Next, A549 cells were transfected with NC (negative control) or mPRα-overexpressing vector, and the culture medium was collected for HUVEC culture (marked as NC-CM or mPRα-CM). HUVECs were cultured in different conditioned mediums in the presence or absence of STAT3 inhibitor Stattic under hypoxia (1% O2) and examined for D cell migration by wound healing assay, E tube formation by Tube formation assay, and F VEGF content in the culture medium by ELISA. *P < 0.05, **P < 0.01, compared to NC or si-NC-CM; #P < 0.05, ##P < 0.01 compared to mPRα-CM

Back to article page