Fig. 2

mPRα activates cAMP/JAK/STAT3 signaling pathway in LUAD cells A549 and PC-9 cells were transfected with si-mPRα or mPRα and examined for (A) cAMP concentrations using a cAMP Direct Immunoassay kit; B VEGF concentrations using ELISA; C the protein levels of p-STAT3, STAT3, VEGF, and HIF1α. Next, A549 and PC-9 cells were transfected with mPRα in the presence or absence of STAT3 inhibitor Stattic (5 μM for 24 h) and examined for (D) VEGF concentrations using ELISA and E the protein levels of p-STAT3, STAT3, VEGF, and HIF1α. *P < 0.05, **P < 0.01, compared to si-NC; #P < 0.05, ##P < 0.01, ###P < 0.005 compared to NC group; $$ P < 0.01, compared to mPRα