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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Prediction of occult tumor progression via platelet RNAs in a mouse melanoma model: a potential new platform for early detection of cancer

Fig. 2

Distinct platelet RNA profiles of mice inoculated with a suboptimal number of B16F10 cells. a Animal model and platelet mRNA sequencing workflow, as starting from B16F10 cell injection, terminal blood collection, to platelet isolation, and mRNA sequencing. C group: negative control group, mice injected with HBSS (Hank's Balanced Salt Solution); S group: suboptimal inoculation group, mice inoculated with 2 × 103 B16F10 cells; O group, optimal inoculation group, mice inoculated with 1 × 105 B16F10 cells (a–d). b Correlation plots of mRNAs detected in platelets of S group, C group and O group mice, including highlighted increased (red) and decreased (blue) platelet mRNAs. NRC, normalized read counts (mean of group). r value calculated from Pearson's correlation test. c Venn diagram of differentially expressed genes from pairwise comparisons. d Heatmap of hierarchical clustering of platelet mRNA profiles of S group (beige), C group (green) and O group (orange). Data pooled (b, c) from n = 5 biologically independent experiments with n = 50 (S group) or n = 40 (C group) and n = 20 (O group) mice, or data representing all 5 independent experiments (d)

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