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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cells

Fig. 4

MTX-NPs regulate the number of Th17/Treg cells and expression of inflammatory cytokines in vivo. Beginning 3 weeks after the first immunization with type II collagen (CII), mice were injected subcutaneously with vehicle, free MTX or MTX-NPs twice per week for 7 weeks (n = 5/group). a–c At 70 days after the first immunization, spleen tissues were isolated and stained for CD4 + IL-17 + (a), CD4 + p-STAT3 (Y705) + (b), and CD4 + CD25 + Foxp3 + (c) cells. Cell subsets were analyzed in four independent quadrants by confocal laser microscopy. The distributions of the cell populations are shown. d At 70 days after the first immunization, ex vivo splenocytes were isolated and the mRNA levels of IL-6, IL-1β, and IL-17 were determined by real-time PCR. Data are means ± SEM of two independent experiments. *P < 0.05 vs. control group

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