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Fig.Ā 2 | Journal of Translational Medicine

Fig.Ā 2

From: Single-cell transcriptomic analysis reveals a novel cell stateĀ and switching genes during hepatic stellate cell activation in vitro

Fig.Ā 2

Simulation of the differentiation trajectory of HSC and the analysis of gene expression pattern. A Trajectory reconstruction of all single cells reveals two branches. HSCs are colored by states, which denote branches and can be used to extract paths. Path 1 consists of the cells in states 1 and 2, while path 2 contains the cells in the states 1 and 3. B HSCs are colored by pseudotime, showing cells developing from state 1 to the bifurcation point that gives rise to the final state 2 and 3, respectively. C HSCs harvested at day 0, 1, 3, 7, and 9 are plotted along pseudotemporal trajectories. D The branched heatmap shows the dynamics of top 250 differentially expressed genes between the two cell fate branches during HSC transdifferentiation. Genes (rows) are divided into four clusters and cells (columns) are ordered according to the pseudotime trajectory. E, F Expression patterns of selected genes between two cell fate branches. The full line represents branch 1 (state 1 and 2) while dotted line represents branch 2 (state 1 and 3)

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