Fig. 6
WT1 activated the AKT/mTOR signaling pathway and ERK/P65 signaling pathway in BRAFV600E PTC cells. A The differentially expressed genes (DEGs) in IHH4 cells treated with vector lentivirus and shWT1 lentivirus. B KEGG analysis indicating the signaling pathways associated with DEGs in IHH4 cells. C Go functional analysis showed the 20 most regulatory signaling pathways associated with DEGs in IHH4 cells by Metascape database. D The protein–protein interaction (PPI) network indicated the directly regulated molecules of WT1, as determined with the GeneMANIA database. E, F GSEA showing that higher WT1 expression led to upregulated signaling pathways in thyroid cancer patients. G–J Western blot analysis showing that WT1 silencing decreased the essential components of AKT/mTOR cascades and the ERK/P65 signaling pathway in IHH4 and BCPAP cells. K, L Quantification of protein expression of the essential components of AKT/mTOR cascades and the ERK/P65 signaling pathway in WT1-silencing IHH4 and BCPAP cells