Fig. 5
Gene knockdown of WT1 regulated autophagy and apoptosis of BRAFV600E PTC cells. A, B Transmission electron microscopy was performed to observe the numbers of autophagic vacuoles, including autophagosomes and autolysosomes, in IHH4 and BCPAP cells after WT1 inhibition. C, D Flow cytometry analysis showing that WT1 inhibition promoted the apoptosis of IHH4 and BCPAP cells. E, F Western blot analysis showing the protein changes in LC3A/B, P62, ATG5 and ATG7 in IHH4 and BCPAP cells with WT1 knocked down. G, H Western blot assay showing that WT1 knockdown modulated the protein expression levels of cleaved caspase 3, BCL2 and BAX in IHH4 and BCPAP cells. I, J Quantification of protein expression of ATG7, ATG5, P62, LC3A/B, BCL2, BAX and cleaved caspase 3 in WT1-silencing IHH4 cells and BCPAP cells (*P < 0.05, ***P < 0.001)