Fig. 5

Analysis of extracellular matrix protein deposition in hepatic melanoma metastases. A Immunofluorescences of Collagen IV and DAPI of livers with hepatic melanoma metastases of B16F10 luc2, RET, WT31, HCmel12 and D4M. White dotted lines present the border of hepatic metastases to adjacent liver tissue. Scale bars = 100 µm, n ≥ 5. B Images of immunofluorescence stainings for Fibronectin and DAPI of liver metastases of B16F10 luc2, RET, WT31, HCmel12 and D4M melanoma. White dotted lines present the border of hepatic metastases to adjacent liver tissue. Scale bars = 100 µm, n ≥ 5. C Immunofluorescences for Lama4 of B16F10 luc2, RET, WT31, HCmel12 and D4M melanoma liver metastases. White dotted lines present the border of hepatic metastases to adjacent liver tissue. Scale bars = 100 µm, n ≥ 5. D Immunofluorescences for Desmin of hepatic B16F10 luc2, RET, WT31, HCmel12 and D4M melanoma metastases. White dotted lines present the border of hepatic metastases to adjacent liver tissue. Scale bars = 100 µm, n ≥ 5. E Pictures of immunofluorescence stainings for Collagen IV, CD31 and DAPI of hepatic melanoma metastases of B16F10 luc2, RET, WT31, HCmel12 and D4M. White dotted lines present the border of hepatic metastases to adjacent liver tissue. Besides, white arrows highlight structures indicative of empty sleeves. Scale bars = 100 µm, n ≥ 5