Fig. 6

The effect of miR-875-5p silencing on GC cells was partially offset by USF2 knockdown. A CCK-8 assays were performed to determine that USF2 knockdown could partly counteract miR-875-5p silencing-induced proliferation in AGS and MKN-45 cells. B EdU assays were performed to determine that USF2 knockdown could partly counteract miR-875-5p silencing-induced proliferation in MKN-45 and AGS cells. C Western blot analysis showed changes in related proteins, such as USF2, Cyclin D1, P21 and P57, in AGS and MKN-45 cells after transfection. All experiments were repeated three times. *p < 0.05; Data expressed as mean ± SD